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ClinMax™ Human Perforin ELISA Kit

For research use only.
    Product Details
    ClinMax™ Human Perforin ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human Perforin that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human Perforin in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
     Perforin Workflow
    Key Features
    AnalytePerforin
    Assay TypeSandwich-ELISA
    ReactivityHuman
    Sensitivity60pg/mL
    Range62.5 pg/mL-2000 pg/mL
    Assay Time2.75 hr
    Sample TypeCell Culture Supernatants, Plasma, Serum.
    Sample volume100 uL
    Format96-wells plate breakable into 12 x 8 wells strips

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 Perforin TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Perforin is less than 60 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 3 samples containing different Perforin concentrations were tested in one assay. Acceptable criteria: CV<10%.

 Perforin INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples containing different concentrations of Perforin were tested in independent assays. Acceptable criteria: CV<15%.

 Perforin INTER-ASSAY STATISTICS
Materials Provided
IDComponentsSize
CEA034-C01Pre-coated Anti-Perforin Antibody Microplate1 plate
CEA034-C02Human Perforin Standard500 μL×2
CEA034-C03Biotin-Anti-Perforin Antibody Con. Solution150 μL
CEA034-C04Biotin-Antibody Dilution Buffer15 mL
CEA034-C05Streptavidin-HRP Con. Solution1.5 mL
CEA034-C06Streptavidin-HRP Dilution Buffer15 mL
CEA034-C0720× Washing Buffer50 mL
CEA034-C08Sample Dilution Buffer15 mL×2
CEA034-C09Substrate Solution12 mL
CEA034-C10Stop Solution6 mL
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 139,440

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