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SeqAssure™ Mass Spectrometry-Verified Recombinant Proteins
High-resolution mass spectrometry enables in-depth protein characterization, providing precise measurement of intact molecular mass and detailed insights into amino acid sequence features. It is a powerful tool for verifying recombinant protein identity and characterizing molecular arttributes.
ACROBiosystems now offers the SeqAssure™ Mass Spectrometry-Verified Recombinant Protein. Building on our established multidimensional physicochemical and functional quality control system, SeqAssure™ adds mass spectrometry data to provide direct, molecular-level evidence of protein identity.
This integrated characterization framework covers both overall molecular properties and detailed sequence features, delivering more comprehensive, traceable, and transparent quality data to meet the stringent requirements of biopharmaceutical R&D and commercialization.
The SeqAssure™ portfolio is continuously expanding. Stay tuned for more.
SeqAssure™ | Mass Spectrometry Verification for Greater Confidence in Quality
Accurate Intact Molecular Mass
Intact proteins are ionized and analyzed by mass spectrometry. Deconvolution of multiply charged ion envelopes determines the molecular mass or mass distribution, enabling direct comparison with the theoretical value.
Measured Molecular Mass
ESI‑MS analysis of Human PTK7, His Tag (Cat. No. PT7-H52H3), showing a major peak at 76520 Da, consistent with the theoretical molecular weight.
High Amino Acid Sequence Coverage
Proteins are enzymatically digested and analyzed by LC-MS/MS peptide mapping. Identified peptides are matched against the target sequence to determine sequence coverage, providing direct evidence of sequence identity.
Sequence Coverage Map
| Proteins | Number of MS Peaks | MS Peak Area | Sequence Coverage | Abundance (mol) |
|---|---|---|---|---|
| B71-H5228 | 278 | 100% | 100% | 100% |
Human B7-1, His Tag (Cat. No. B71-H5228) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage.
Confirmed N-Terminal Start Site
N-terminal sequencing determines the first 10–15 amino acid residues of the protein. Comparison with the expected sequence confirms the N-terminal start site and verifies that expression begins at the correct position.
N-terminal sequencing
N-terminal sequencing of Human CD117 (Cat. No. CD7-H52H4) confirmed that the experimentally determined N-terminal sequence is consistent with the theoretical starting sequence.
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